INTRODUCTION: FM are gaining wide acceptance for use in measuring regional BF. This study was designed to evaluate inter-animal variation in baseline BF and intra-animal variation based on paired organ BF comparisons in normal rats. METHODS: Male S-D rats (350-450g, n=33) were anesthetized with ketamine and catheters (PE-50) were advanced into the left ventricle (LV) and abdominal aorta caudal to the renal arteries. Blue-green FM (0.2x106 spheres in 0.2 ml) were injected into the LV over 3s followed by a 0.2 ml saline flush. Reference blood was withdrawn at a rate of 0.5 ml/min 5s prior to FM injection and 30s following the flush. Tissues were digested in 4% KOH, FM extracted using vacuum filtration and dye extracted using Carbitol acetate. RESULTS: BF - ml/min/100g ww, mean±SE.
|L Lung||58±12||R Adrenal||534±62||Diaphragm||49±4|
|R Lung||71±13||Duodenum||310±18||Soleus m||10±1|
CONCLUSIONS: FM can give reliable organ BF values during normal hemodynamic function in rats. The similarity between paired organs confirms the uniformity of FM suspensions as well as adequate mixing in the LV. Reliability of this technique during low-flow states in rats remains to be determined.
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