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Heart Composite Picture
Processing of fluorescent images.
  • A. 2000 x 2000 pixel outline image of rabbit heart en face.
  • B. Bitmap image (black = 0 and white = 1) defining the spatial location of heart tissue
  • C. Fluorescent image obtained with a specific excitation/emission filter pair to determine individual microsphere location. Each point represents a yellow microsphere located by an x, y, and z (slice) location. The spatial coordinates and cluster size of each microsphere is written to a text file.
  • D. Three-dimensional distribution of 26083 yellow microspheres in a rabbit heart